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Product Name
c-SRC antibody
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Description
c-SRC Mouse Monoclonal antibody. Positive WB detected in CHO cells. Positive IHC detected in human breast cancer tissue. Positive IF detected in MCF-7 cells. Observed molecular weight by Western-blot: 60 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, hamster; other species not tested.
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Alternative names
ASV antibody; c SRC antibody; c-SRC antibody; p60 Src antibody; pp60c src antibody; Proto oncogene c Src antibody; SRC antibody; SRC1 antibody
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Isotype
Mouse IgG2b
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Preparation
This antibody was obtained by immunization of c-SRC recombinant protein (Accession Number: XM_047440405). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
CHO cells were subjected to SDS PAGE followed by western blot with Catalog No:107180(c-SRC Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:107180(c-SRC Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:107180(c-SRC Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed MCF-7 cells using Catalog No:107180(c-SRC Antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
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Background
SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase.
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References
- Yang F, Yu X, Liu C. Phospho-selective mechanisms of arrestin conformations and functions revealed by unnatural amino acid incorporation and (19)F-NMR. Nature communications. 6:8202. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"