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Product Name
c-Myb antibody
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Description
c-Myb Rabbit Polyclonal antibody. Positive WB detected in HL-60 cells, Jurkat cells. Positive IP detected in HL-60 cells. Observed molecular weight by Western-blot: 72-80kd
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
c myb_CDS antibody; Cmyb antibody; c-Myb antibody; efg antibody; MYB antibody; Proto oncogene c Myb antibody; Transcriptional activator Myb antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of c-Myb recombinant protein (Accession Number: NM_005375). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:1000
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Validations
HL-60 cells were subjected to SDS PAGE followed by western blot with Catalog No:109417(MYB antibody) at dilution of 1:400
IP Result of anti-MYB (IP:Catalog No:109417, 4ug; Detection:Catalog No:109417 1:300) with HL-60 cells lysate 4000ug.
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Background
The MYB protein is a transcription factor that plays a key role in regulating the proliferation/differentiation of progenitor cells in bone marrow, colonic crypts, and neurogenic niches [PMID:18574464]. It is involved in the regulation of epithelial-to-mesenchymal transition (EMT) and invasion in neuroblastoma, colon carcinoma, and embryonic kidney cells through the upregulation of the transcription repressor Slug [PMID:20622260]. It also a DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3', and plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"