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Product Name
BUB1 antibody
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Description
BUB1 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, SGC-7901 cells. Positive IF detected in IMR-90 cells , MDCK cells. Observed molecular weight by Western-blot: 45 kDa
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Tested applications
ELISA, IF, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
BUB1 antibody; BUB1A antibody; BUB1L antibody; hBUB1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of BUB1 recombinant protein (Accession Number: NM_004336). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IF: N/A
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Validations
IF results of BUB1 anitbody with IMR-90 cells by M.S. Grasiella Angelina Andriani Cristina Montagna Lab at Albert Einstein College of Medicine-Bronx - NY.
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:108551(BUB1 Antibody) at dilution of 1:600
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Background
Bub1 is an evolutionarily conserved mitotic checkpoint control protein that is present in diverse organisms including yeast and humans. Bub1 is a serine/threonine protein kinase and is required for recruitment of Mad1, Mad2, Bub3, and CENP-E to kinetochores. Full-length Bub1 protein was cleaved to an 45 kDa fragment and an intermediate fragment of 90 kDa, only detected at early stages of apoptosis execution, indicating cleavage of Bub1 in the amino- and carboxy-terminal regions of the protein(PMID:15818396).
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