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Product Name
BRSK1 antibody
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Description
BRSK1 Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue, human brain tissue. Positive IP detected in mouse brain tissue. Positive IHC detected in human ovary tumor tissue. Observed molecular weight by Western-blot: 85-90 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
BR serine/threonine kinase 1 antibody; BRSK1 antibody; FLJ43009 antibody; KIAA1811 antibody; SAD1 antibody; SAD1 kinase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of BRSK1 recombinant protein (Accession Number: BC016681). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:117244(BRSK1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:117244(BRSK1 antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-BRSK1 (IP:Catalog No:117244, 4ug; Detection:Catalog No:117244 1:500) with mouse brain tissue lysate 3440ug.
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Background
BRSK1(Brain-specific serine/threonine-protein kinase 1) is also named as KIAA1811, SAD1, SADB and belongs to the SNF1 subfamily. BRSKs are crucial for establishing neuronal polarity, and BRSK1 has also been shown to regulate neurotransmitter release presynaptically. BRSK1 is a serine-threonine kinases specifically expressed in the brain, and activated by LKB1-mediated phosphorylation of a threonine residue at their T-loop. (PMID:22020259). It has 3 isoforms produced by alternative splicing.
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References
- Morita T, Sobue K. Specification of neuronal polarity regulated by local translation of CRMP2 and Tau via the mTOR-p70S6K pathway. The Journal of biological chemistry. 284(40):27734-45. 2009.
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