BRD2 antibody

Cat.#: 108580

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Product Information

  • Product Name
    BRD2 antibody
  • Documents
  • Description
    BRD2 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive WB detected in HeLa cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 100-110 kDa
  • Tested applications
    ELISA, WB, IP, IF
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    BRD2 antibody; bromodomain containing 2 antibody; D6S113E antibody; DKFZp686N0336 antibody; FLJ31942 antibody; FSH antibody; FSRG1 antibody; KIAA9001 antibody; NAT antibody; O27.1.1 antibody; RING3 antibody; RNF3 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of BRD2 recombinant protein (Accession Number: NM_001199455). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:1000

    IF: 1:50-1:500

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:117227(BRD2 Antibody) at dilution of 1:600

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:117227(BRD2 Antibody) at dilution of 1:600

    IP Result of anti-BRD2 (IP:Catalog No:117227, 4ug; Detection:Catalog No:117227 1:300) with HeLa cells lysate 1200ug.

    IP Result of anti-BRD2 (IP:Catalog No:117227, 4ug; Detection:Catalog No:117227 1:300) with HeLa cells lysate 1200ug.

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:117227(BRD2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:117227(BRD2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    BRD2 represents bromodomain containing 2

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"