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Product Name
BRCC3 antibody
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Description
BRCC3 Rabbit Polyclonal antibody. Positive IHC detected in human ovary tissue, human brain tissue. Positive WB detected in MCF7 cells, COLO 320 cells, COS-7 cells, HEK-293 cells, mouse skeletal muscle tissue, SH-SY5Y cells. Positive IP detected in mouse heart tissue. Observed molecular weight by Western-blot: 65-70kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat, Monkey; other species not tested.
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Alternative names
BRCA1 A complex subunit BRCC36 antibody; BRCC3 antibody; BRCC36 antibody; BRISC complex subunit BRCC36 antibody; C6.1A antibody; CXorf53 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of BRCC3 recombinant protein (Accession Number: NM_024332). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:117226(BRCC3 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human ovary using Catalog No:117226(BRCC3 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human ovary using Catalog No:117226(BRCC3 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-BRCC3 (IP:Catalog No:117226, 4ug; Detection:Catalog No:117226 1:400) with mouse heart tissue lysate 4000ug.
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Background
BRCC3 represents BRCA1/BRCA2-containing complex, subunit 3
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"