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Product Name
BRAF antibody
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Description
BRAF Rabbit Polyclonal antibody. Positive IF detected in NIH/3T3 cells. Positive IHC detected in human thyroid cancer tissue, human testis tissue.
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Tested applications
ELISA, IHC, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
B RAF1 antibody; BRAF antibody; BRAF1 antibody; FLJ95109 antibody; p94 antibody; Proto oncogene B Raf antibody; RAFB1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of BRAF recombinant protein (Accession Number: NM_004333). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
Immunohistochemistry of paraffin-embedded human thyroid cancer tissue slide using Catalog No:117222(BRAF Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human thyroid cancer tissue slide using Catalog No:117222(BRAF Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using Catalog No:117222(BRAF Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
BRAF represents v-raf murine sarcoma viral oncogene homolog B1
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"