BNIP1 antibody

BNIP1 antibody

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BNIP1 Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in human brain tissue, HeLa cells, human liver tissue, mouse skeletal muscle tissue, rat brain tissue. Observed molecular weight by Western-blot: 26 kDa

ELISA, WB, IP

Human,Mouse,Rat; other species not tested.

BNIP1 antibody; NIP1 antibody; SEC20 antibody; SEC20L antibody; TRG 8 antibody

Rabbit IgG

This antibody was obtained by immunization of BNIP1 recombinant protein (Accession Number: NM_001205). Purification method: Antigen affinity purified.

Polyclonal

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Store at -20℃. DO NOT ALIQUOT

BNIP1 is a member of the BCL2/adenovirus E1B 19 kDa-interacting protein (BNIP) family. The encoded protein is predominantly localized to the endoplasmic reticulum (ER), is a pro-apoptotic Bcl-2 homology domain 3 (BH3)-only protein. BNIP1, also called SEC20L, is a component of a SNARE complex consisting of STX18, USE1L, BNIP1/SEC20L and SEC22B which is involved in apoptosis and ER membrane fusion. Recent reports showed that expression of BNIP1 induced mitochondrial fragmentation in a BH3 domain-dependent manner via increasing dynamin-related protein 1 (Drp1) expression. RNF185 is a mitochondrial ubiquitin E3 ligase that regulates selective mitochondrial autophagy, BNIP1 colocalizes with RNF185 at mitochondria and is polyubiquitinated by RNF185 through K63-based ubiquitin linkage in vivo and modulatemitochondrial homeostasis through autophagy.

• Tang F, Wang B, Li N. RNF185, a novel mitochondrial ubiquitin E3 ligase, regulates autophagy through interaction with BNIP1. PloS one. 6(9):e24367. 2011.
• Wang P, Wu Y, Li Y, Zheng J, Tang J. A novel RING finger E3 ligase RNF186 regulate ER stress-mediated apoptosis through interaction with BNip1. Cellular signalling. 25(11):2320-33. 2013.