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BMP2 antibody

Cat.#: 108461

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Product Information

  • Product Name
    BMP2 antibody
  • Documents
  • Description
    BMP2 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HL-60 cells, HT-1080 cells, mouse lung tissue, PC-13 cells, Sp2/0 cells. Positive IHC detected in human small intestine tissue, human colon tissue, human heart tissue, human lung tissue. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 45 kDa
  • Tested applications
    ELISA, WB, IHC, IF
  • Species reactivity
    Human,Mouse,Rat,Pig; other species not tested.
  • Alternative names
    BMP 2 antibody; BMP 2A antibody; BMP2 antibody; BMP2A antibody; bone morphogenetic protein 2 antibody; Bone morphogenetic protein 2A antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of BMP2 recombinant protein (Accession Number: BC069214). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:50-1:500

  • Validations

    Immunohistochemistry of paraffin-embedded human small intestine tissue slide using Catalog No:117196(BMP2 Antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemistry of paraffin-embedded human small intestine tissue slide using Catalog No:117196(BMP2 Antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemistry of paraffin-embedded human small intestine tissue slide using Catalog No:117196(BMP2 Antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemistry of paraffin-embedded human small intestine tissue slide using Catalog No:117196(BMP2 Antibody) at dilution of 1:50 (under 40x lens)

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:117196(BMP2 antibody) at dilution of 1:1000

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:117196(BMP2 antibody) at dilution of 1:1000

    Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:117196(BMP2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:117196(BMP2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    Bone morphogenetic protein-2 (BMP-2) is a member of the transforming growth factor-beta (TGFB) superfamily. BMP2 is synthesized as a 60 kDa precursor that is processed in the secretory pathway to a small 18 kDa monomer; 2 monomers then associate to form the active 30 kDa homodimer, which binds to its receptor. There is also a 40-45 kDa form of BMP2, as an amino-terminal propeptide. BMP2 can induce bone formation and regeneration during early embryonic development. It is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction.
  • References
    • Wang XY, Chen ZH, Zhang RY. Construction of a eukaryotic expression vector pEGFP-C1-BMP-2 and its effect on cell migration. Journal of Zhejiang University. Science. B. 13(5):356-63. 2012.
    • Chen LP, Li ZF, Ping M. Regulation of Olig2 during astroglial differentiation in the subventricular zone of a cuprizone-induced demyelination mouse model. Neuroscience. 221:96-107. 2012.
    • Xu D, Yang W, Hu Y. Surface functionalization of titanium substrates with cecropin B to improve their cytocompatibility and reduce inflammation responses. Colloids and surfaces. B, Biointerfaces. 110:225-35. 2013.
    • Wei Y, Wu Y, Zeng B, Zhang H. Effects of sodium fluoride treatment in vitro on cell proliferation, BMP-2 and BMP-3 expression in human osteosarcoma MG-63 cells. Biological trace element research. 162(1-3):18-25. 2014.
    • Gu GJ, Chen T, Zhou HM, Sun KX, Li J. Role of Wnt/β-catenin signaling pathway in the mechanism of calcification of aortic valve. Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban. 34(1):33-6. 2014.
    • Liu T, Zou W, Shi G. Hypoxia-induced MTA1 promotes MC3T3 osteoblast growth but suppresses MC3T3 osteoblast differentiation. European journal of medical research. 20:10. 2015.
    • Zhou Y, Wang JY, Feng H. Overexpression of c1q/tumor necrosis factor-related protein-3 promotes phosphate-induced vascular smooth muscle cell calcification both in vivo and in vitro. Arteriosclerosis, thrombosis, and vascular biology. 34(5):1002-10. 2014.
    • Zhang X, Xiao J, Li R. Metformin alleviates vascular calcification induced by vitamin D3 plus nicotine in rats via the AMPK pathway. Vascular pharmacology. 2016.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"