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Product Name
Biglycan antibody
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Description
Biglycan Rabbit Polyclonal antibody. Positive IHC detected in human lung tissue, human liver cancer tissue, human renal cell carcinoma tissue, human stomach cancer tissue, mouse lung tissue. Positive IF detected in HepG2 cells. Positive IP detected in mouse skeletal muscle tissue. Positive WB detected in COLO 320 cells, HeLa cells, HepG2 cells, mouse skeletal muscle tissue, pig cartilage tissue. Observed molecular weight by Western-blot: 40-48 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
A6NLG9 antibody; BGN antibody; biglycan antibody; Bone/cartilage proteoglycan I antibody; DSPG1 antibody; PG S1 antibody; PGI antibody; SLRR1A antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Biglycan recombinant protein (Accession Number: NM_001711). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
COLO 320 cells were subjected to SDS PAGE followed by western blot with Catalog No:117144(BGN antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human lung using Catalog No:117144(BGN antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human lung using Catalog No:117144(BGN antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-Biglycan (IP:Catalog No:117144, 4ug; Detection:Catalog No:117144 1:300) with mouse skeletal muscle tissue lysate 2200ug.
Immunofluorescent analysis of HepG2 cells using Catalog No:117144(Biglycan Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
BGN represents biglycan
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"