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Product Name
Bif-1 antibody
- Documents
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Description
Bif-1 Rabbit Polyclonal antibody. Positive WB detected in mouse kidney tissue, HEK-293 cells, human placenta tissue, mouse heart tissue, mouse placenta tissue. Positive IHC detected in human kidney tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 41kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Bax interacting factor 1 antibody; Bif 1 antibody; Bif1 antibody; Endophilin B1 antibody; SH3GLB1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Bif-1 recombinant protein (Accession Number: NM_016009). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:117143(SH3GLB1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human kidney using Catalog No:117143(SH3GLB1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:117143(SH3GLB1 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using SH3GLB1 antibody Catalog No:117143 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
SH3GLB1 represents SH3-domain GRB2-like endophilin B1
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