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Product Name
BBS9 antibody
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Description
BBS9 Rabbit Polyclonal antibody. Positive IP detected in mouse testis tissue. Positive WB detected in mouse testis tissue, human heart tissue. Positive IF detected in hTERT-RPE1 cells. Positive IHC detected in human liver tissue. Observed molecular weight by Western-blot: 60 kDa
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Tested applications
ELISA, IHC, IF, WB, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
Bardet Biedl syndrome 9 antibody; BBS9 antibody; C18 antibody; Protein PTHB1 antibody; PTHB1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of BBS9 recombinant protein (Accession Number: BC032715). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:108377(BBS9 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human liver using Catalog No:108377(BBS9 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:108377(BBS9 antibody) at dilution of 1:50 (under 40x lens)
IF result (cytoplasm stain) of anti-BBS9 (Catalog No:108377; 1:50) with hTERT-RPE1 cell by Dr. Moshe Kim.
IP Result of anti-BBS9 (IP:Catalog No:108377, 4ug; Detection:Catalog No:108377 1:500) with mouse testis tissue lysate 4000ug.
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Background
BBS9 represents Bardet-Biedl syndrome 9
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References
- Sinha S, Belcastro M, Datta P, Seo S, Sokolov M. Essential role of the chaperonin CCT in rod outer segment biogenesis. Investigative ophthalmology & visual science. 55(6):3775-85. 2014.
- Eguether T, San Agustin JT, Keady BT. IFT27 links the BBSome to IFT for maintenance of the ciliary signaling compartment. Developmental cell. 31(3):279-90. 2014.
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