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Product Name
BBS4 antibody
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Description
BBS4 Rabbit Polyclonal antibody. Positive IF detected in hTERT-RPE1 cells. Positive IHC detected in human kidney tissue. Positive WB detected in HEK-293 cells, HeLa cells, mouse kidney tissue. Observed molecular weight by Western-blot: 50-60 kDa
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Tested applications
ELISA, IHC, IF, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Bardet Biedl syndrome 4 antibody; BBS4 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of BBS4 recombinant protein (Accession Number: NM_033028). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:108371(BBS4 antibody) at dilution of 1:500
IF result (cytoplasm and the length of cilia cilia stain) of anti-BBS4 (Catalog No:108371; 1:50) with serum-starved hTERT-RPE1 cell (MeOH fixed) by Dr. Moshe Kim.
Immunohistochemical of paraffin-embedded human kidney using Catalog No:108371(BBS4 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:108371(BBS4 antibody) at dilution of 1:50 (under 40x lens)
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Background
BBS4 represents Bardet-Biedl syndrome 4
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References
- Xu Q, Zhang Y, Wei Q. BBS4 and BBS5 show functional redundancy in the BBSome to regulate the degradative sorting of ciliary sensory receptors. Scientific reports. 5:11855. 2015.
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