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Product Name
BARHL2 antibody
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Description
BARHL2 Rabbit Polyclonal antibody. Positive IHC detected in human testis tissue. Positive IF detected in HepG2 cells. Positive WB detected in HepG2 cells, NIH/3T3 cells. Observed molecular weight by Western-blot: 42-45 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
BarH like 2 homeobox protein antibody; BarH like homeobox 2 antibody; BARHL2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of BARHL2 recombinant protein (Accession Number: NM_020063). Purification method: Antigen Affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:108359(BARHL2 Antibody) at dilution of 1:300
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:108359(BARHL2 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:108359(BARHL2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
BARHL2 also named as BarH like homeobox 2 is 279 amino acid protein, which contains 1 homeobox DNA-binding domain. BARHL2 localizes in nucleus and belongs to BAR homeobox family. BARHL2 as a transcription factor binds optimally to the DNA consensus sequence. Barx2 may differentially control the expression of L1 and other target genes during embryonic development. Barx2 is expressed in several smooth muscle-containing tissues, as well as skeletal muscle, brain, tongue and esophagus.
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