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Product Name
BAF170 antibody
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Description
BAF170 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human medulloblastoma tissue. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, A431 cells. Observed molecular weight by Western-blot: 170 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
BAF170 antibody; BRG1 assOCiated factor 170 antibody; CRACC2 antibody; Rsc8 antibody; SMARCC2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of BAF170 recombinant protein (Accession Number: NM_139067). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:108405(SMARCC2 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human medulloblastoma using Catalog No:108405(SMARCC2 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using SMARCC2 antibody Catalog No:108405 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-BAF170 (IP:Catalog No:108405, 4ug; Detection:Catalog No:108405 1:700) with HeLa cells lysate 3200ug.
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Background
SMARCC2 represents SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2
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