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Product Name
BAD antibody
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Description
BAD Rabbit Polyclonal antibody. Positive IHC detected in human prostate hyperplasia tissue, human lymphoma tissue. Positive WB detected in mouse heart tissue, Tunicamycin treated HeLa cells. Observed molecular weight by Western-blot: 15-18 kDa; 25 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
BAD antibody; BBC2 antibody; BBC6 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of BAD recombinant protein (Accession Number: NM_004322). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:108404(BAD antibody) at dilution of 1:300
Tunicamycin treated HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:108404(BAD Antibody) at dilution of 1:300
Immunohistochemistry of paraffin-embedded human prostate hyperplasia tissue slide using Catalog No:108404(BAD Antibody) at dilution of 1:200 (under 10x lens)
Immunohistochemistry of paraffin-embedded human prostate hyperplasia tissue slide using Catalog No:108404(BAD Antibody) at dilution of 1:200 (under 40x lens)
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Background
BAD, also named as BBC6 and BCL2L8, promotes cell death. It is successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. BAD can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. It appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
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References
- Yu G, Jiang L, Xu Y. Silencing prion protein in MDA-MB-435 breast cancer cells leads to pleiotropic cellular responses to cytotoxic stimuli. PloS one. 7(11):e48146. 2012.
- Zhao L, Teng B, Wen L. mTOR inhibitor AZD8055 inhibits proliferation and induces apoptosis in laryngeal carcinoma. International journal of clinical and experimental medicine. 7(2):337-47. 2014.
- Wang J, Lao L, Zhao H, Huang Y. Serine threonine kinase Pim-3 regulates STAT3 pathway to inhibit proliferation of human liver cancers. International journal of clinical and experimental medicine. 7(2):348-55. 2014.
- Lu Q, Lv M, Xu E. Recombinant hirudin suppresses the viability, adhesion, migration and invasion of Hep-2 human laryngeal cancer cells. Oncology reports. 33(3):1358-64. 2015.
- Dai B, Wang W, Liu R, Wang H, Zhang Y. Novel taspine derivative 12k inhibits cell growth and induces apoptosis in lung cell carcinoma. Biomedicine & pharmacotherapy = Biomédecine & pharmacothérapie. 70:227-33. 2015.
- Xie HX, Xu ZY, Tang JN. Effect of Huaier on the proliferation and apoptosis of human gastric cancer cells through modulation of the PI3K/AKT signaling pathway. Experimental and therapeutic medicine. 10(3):1212-1218. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"