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Product Name
BACE1 antibody
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Description
BACE1 Rabbit Polyclonal antibody. Positive IHC detected in human pancreas cancer tissue. Positive WB detected in mouse brain tissue, HeLa cells, human brain tissue, mouse pancreas tissue, zebrafish tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 70-75 kDa
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Tested applications
ELISA, WB, IP, IHC
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Species reactivity
Human, Mouse, Zebrafish; other species not tested.
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Alternative names
Asp 2 antibody; ASP2 antibody; Aspartyl protease 2 antibody; BACE antibody; BACE1 antibody; Beta secretase 1 antibody; FLJ90568 antibody; HSPC104 antibody; KIAA1149 antibody; Memapsin 2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of BACE1 recombinant protein (Accession Number: NM_012104). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:108401(BACE1 antibody) at dilution of 1:500
IP Result of anti-BACE1 (IP:Catalog No:108401, 3ug; Detection:Catalog No:108401 1:500) with mouse brain tissue lysate 7500ug.
Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using Catalog No:108401(BACE1 Antibody) at dilution of 1:50 (under 40x lens)
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Background
beta-secretase, encoded by BACE1(beta-stie amyloid beta A4 precursor protein-cleavage enzyem1) gene, participate in the two step proteolytic processing of the APP. Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has a high expression in the brain and pancreas. This protein has 6 isoforms produced by alternative splicing. BACE1 can exsit as a homodimer with the MW of 120 kDa and the glycosylated immature with the MW of 75 kDa.(PMID:22741101; 21795680).
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References
- Joshi G, Chi Y, Huang Z, Wang Y. Aβ-induced Golgi fragmentation in Alzheimer's disease enhances Aβ production. Proceedings of the National Academy of Sciences of the United States of America. 111(13):E1230-9. 2014.
- Hayakawa M, Itoh M, Ohta K. Quercetin reduces eIF2α phosphorylation by GADD34 induction. Neurobiology of aging. 36(9):2509-18. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"