Product Name

ATPB antibody

Documents

Description

ATPB Rabbit Polyclonal antibody. Positive IP detected in mouse heart tissue. Positive WB detected in human heart tissue, HeLa cells, HepG2 cells, mouse liver tissue, rat heart tissue, rat liver tissue. Positive IF detected in HeLa cells. Positive IHC detected in human liver tissue. Observed molecular weight by Western-blot: 56 kDa

Tested applications

ELISA, WB, IHC, IP, IF

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

ATP5B antibody; ATPB antibody; ATPMB antibody; ATPSB antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of ATPB recombinant protein (Accession Number: NM_001686). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IP: 1:200-1:2000

IHC: 1:20-1:200

IF: 1:20-1:200

Validations

human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:108322(ATPB antibody) at dilution of 1:500

IP Result of anti-ATPB (IP:Catalog No:108322, 3ug; Detection:Catalog No:108322 1:500) with mouse heart tissue lysate 7500ug.

Immunohistochemical of paraffin-embedded human liver using Catalog No:108322(ATPB antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical of paraffin-embedded human liver using Catalog No:108322(ATPB antibody) at dilution of 1:100 (under 40x lens)

Immunofluorescent analysis of HeLa cells using Catalog No:108322(ATPB Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Background

ATP5B, also named as ATPMB and ATPSB, belongs to the ATPase alpha/beta chains family. It is a high affinity HDL receptor for apolipoprotein A1. ATP5B is a subunit of mitochondrial ATP synthase (F1F0 ATP synthase or Complex V ).

References

Preuss B, Berg C, Dengjel J, Stevanovic S, Klein R. Relevance of the inner mitochondrial membrane enzyme F1F0-ATPase as an autoantigen in autoimmune liver disorders. Liver international : official journal of the International Association for the Study of the Liver. 32(2):249-57. 2012.
Hall JA, Kusuma BR, Brandt GE, Blagg BS. Cruentaren A binds F1F0 ATP synthase to modulate the Hsp90 protein folding machinery. ACS chemical biology. 9(4):976-85. 2014.
Jokinen R, Lahtinen T, Marttinen P. Quantitative changes in Gimap3 and Gimap5 expression modify mitochondrial DNA segregation in mice. Genetics. 200(1):221-35. 2015.
Richter U, Lahtinen T, Marttinen P, Suomi F, Battersby BJ. Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness. The Journal of cell biology. 211(2):373-89. 2015.

ATPB antibody

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