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Product Name
ATG12 antibody
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Description
ATG12 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive IF detected in PC-3 cells. Positive WB detected in PC-3 cells, COLO 320 cells, NIH/3T3 cells. Observed molecular weight by Western-blot: 48-55kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
APG12 antibody; APG12 like antibody; APG12L antibody; ATG12 antibody; Autophagy related protein 12 antibody; FBR93 antibody; HAPG12 antibody; Ubiquitin like protein ATG12 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ATG12 recombinant protein (Accession Number: BC012266). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:10-1:100
IF: 1:10-1:100
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Validations
PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:108289(ATG12 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:108289(ATG12 antibody) at dilution of 1:10 (under 10x lens)
Immunofluorescent analysis of PC-3 cells using Catalog No:108289(ATG12 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
ATG12 represents ATG12 autophagy related 12 homolog (S. cerevisiae)
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"