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Product Name
ATG12 antibody
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Description
ATG12 Rabbit Polyclonal antibody. Positive IHC detected in human prostate cancer tissue. Positive IP detected in HEK-293 cells. Positive WB detected in human bladder tissue, UV treated HEK-293 cells. Observed molecular weight by Western-blot: 48-50kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
APG12 antibody; APG12 like antibody; APG12L antibody; ATG12 antibody; Autophagy related protein 12 antibody; FBR93 antibody; HAPG12 antibody; Ubiquitin like protein ATG12 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ATG12 recombinant protein (Accession Number: NM_001277783). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
human bladder tissue were subjected to SDS PAGE followed by western blot with Catalog No:108288(ATG12 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:108288(ATG12 antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-ATG12 (IP:Catalog No:108288, 4ug; Detection:Catalog No:108288 1:300) with HEK-293 cells lysate 880ug.
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Background
ATG12, also named as APG12 and APG12L, belongs to the ATG12 family. It is required for autophagy. Free ATG12 is 15kd or 8kd. Conjugated to ATG5, ATG12-ATG5 show 48-55kd.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"