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Product Name
ATF6B antibody
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Description
ATF6B Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, human testis tissue. Positive IP detected in Jurkat cells. Positive IHC detected in human brain tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 110 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ATF6 beta antibody; ATF6B antibody; CREB RP antibody; CREBL1 antibody; FLJ10066 antibody; G13 antibody; Protein G13 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ATF6B recombinant protein (Accession Number: BC008394). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:108287(ATF6B antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human brain using Catalog No:108287(ATF6B antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using ATF6B antibody Catalog No:108287 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-ATF6B (IP:Catalog No:108287, 4ug; Detection:Catalog No:108287 1:1000) with Jurkat cells lysate 3200ug.
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Background
The endoplasmic reticulum (ER)-transmembrane proteins, ATF6 alpha (ATF6) and ATF6 beta (ATF6B), are cleaved during the ER stress response (ERSR). The resulting N-terminal fragments of both ATF6 isoforms, which have conserved basic leucine-zipper and DNA binding domains but divergent transcriptional activation domains, translocate to the nucleus where they bind to ER stress-response elements (ERSE) in ERSR, such as Bip/GRP78. This antibody could recognize ATF6 beta (110kd) and its cleaved forms(60,80kd )(PMID: 11256944)
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References
- Chen YJ, Su JH, Tsao CY. Sinulariolide induced hepatocellular carcinoma apoptosis through activation of mitochondrial-related apoptotic and PERK/eIF2α/ATF4/CHOP pathway. Molecules (Basel, Switzerland). 18(9):10146-61. 2013.
- Xu T, Zheng L, Xu L. Protective effects of dioscin against alcohol-induced liver injury. Archives of toxicology. 88(3):739-53. 2014.
- Fang JY, Wang PW, Huang CH, Hung YY, Pan TL. Evaluation of the hepatotoxic risk caused by lead acetate via skin exposure using a proteomic approach. Proteomics. 14(21-22):2588-99. 2014.
- Jensen BK, Monnerie H, Mannell MV. Altered Oligodendrocyte Maturation and Myelin Maintenance: The Role of Antiretrovirals in HIV-Associated Neurocognitive Disorders. Journal of neuropathology and experimental neurology. 74(11):1093-118. 2015.
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