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Product Name
Ataxin 2 antibody
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Description
Ataxin 2 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells, Ethacrynic acid treated HepG2 cells. Positive IHC detected in human gliomas tissue. Positive WB detected in HeLa cells, DU 145 cells, HEK-293 cells. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 140-150 kDa
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Tested applications
ELISA, IHC, IF, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ataxin 2 antibody; ATX2 antibody; ATXN2 antibody; FLJ46772 antibody; SCA2 antibody; TNRC13 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Ataxin 2 recombinant protein (Accession Number: NM_001310123). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:108280(ATXN2 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:108280(ATXN2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:108280(ATXN2 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of 293 cells, using ATXN2 antibody Catalog No:108280 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-Ataxin 2 (IP:Catalog No:108280, 4ug; Detection:Catalog No:108280 1:1000) with HEK-293 cells lysate 1200ug.
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Background
ATXN2 contains a repeat structure with 22 or 23 triplets coding for glutamine and the (CAG)8CAA(CAG)4CAA(CAG)8 sequence; expansion of this domain to a size ≥34 triplets with a pure CAG sequence primarily causes autosomal dominant SCA2 [PMID:18418684], while ATXN2 expansions with CAA interruptions were observed as the cause of Levo-dopa responsive Parkinson’s disease [PMID:10668726]. ATXN2 expansions associated with ALS were reported to be interrupted by at least one CAA triplet [PMID:21537950].
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References
- Colombrita C, Onesto E, Megiorni F. TDP-43 and FUS RNA-binding proteins bind distinct sets of cytoplasmic messenger RNAs and differently regulate their post-transcriptional fate in motoneuron-like cells. The Journal of biological chemistry. 287(19):15635-47. 2012.
- Halbach MV, Gispert S, Stehning T, Damrath E, Walter M, Auburger G. Atxn2 Knockout and CAG42-Knock-in Cerebellum Shows Similarly Dysregulated Expression in Calcium Homeostasis Pathway. Cerebellum (London, England). 2016.
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