ASF1B antibody

Cat.#: 108224

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Product Information

  • Product Name
    ASF1B antibody
  • Documents
  • Description
    ASF1B Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, A431 cells. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 19 kDa
  • Tested applications
    ELISA, WB, IF
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    ASF1B antibody; CCG1 interacting factor A II antibody; CIA II antibody; FLJ10604 antibody; hAsf1 antibody; hAsf1b antibody; hCIA II antibody; Histone chaperone ASF1B antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of ASF1B recombinant protein (Accession Number: NM_018154). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IF: 1:20-1:200

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:108224(ASF1B antibody) at dilution of 1:1000

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:108224(ASF1B antibody) at dilution of 1:1000

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:108224(ASF1B Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:108224(ASF1B Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    ASF1B is a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly.This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly. This antibody specifically reacts with the 19kd ASF1B protein.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"