ARPC5L antibody

Cat.#: 108169

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Product Information

  • Product Name
    ARPC5L antibody
  • Documents
  • Description
    ARPC5L Rabbit Polyclonal antibody. Positive FC detected in MCF-7 cells. Positive IHC detected in human placenta tissue, human cerebellum tissue. Positive IF detected in MCF-7 cells. Positive WB detected in mouse brain tissue, human brain tissue, human placenta tissue, mouse lung tissue, NIH/3T3 cells. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 17-20 kDa
  • Tested applications
    ELISA, WB, IHC, IF, FC, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    ARC16 2 antibody; ARPC5L antibody
  • Immunogen
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of ARPC5L recombinant protein (Accession Number: NM_030978). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:108169(ARPC5L antibody) at dilution of 1:1000

    mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:108169(ARPC5L antibody) at dilution of 1:1000

    Immunofluorescent analysis of MCF-7 cells using Catalog No:108169(ARPC5L Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

    Immunofluorescent analysis of MCF-7 cells using Catalog No:108169(ARPC5L Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

    Immunohistochemical of paraffin-embedded human placenta using Catalog No:108169(ARPC5L antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human placenta using Catalog No:108169(ARPC5L antibody) at dilution of 1:50 (under 10x lens)

    1X10^6 MCF-7 cells were stained with 0.2ug ARPC5L antibody (Catalog No:108169, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 MCF-7 cells were stained with 0.2ug ARPC5L antibody (Catalog No:108169, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    IP Result of anti-ARPC5L (IP:Catalog No:108169, 4ug; Detection:Catalog No:108169 1:1000) with mouse brain tissue lysate 4000ug.

    IP Result of anti-ARPC5L (IP:Catalog No:108169, 4ug; Detection:Catalog No:108169 1:1000) with mouse brain tissue lysate 4000ug.

    Immunohistochemical of paraffin-embedded human placenta using Catalog No:108169(ARPC5L antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human placenta using Catalog No:108169(ARPC5L antibody) at dilution of 1:50 (under 40x lens)

  • Background
    ARPC5L represents actin related protein 2/3 complex, subunit 5-like

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"