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Product Name
ARNT,HIF1B antibody
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Description
ARNT,HIF1B Rabbit Polyclonal antibody. Positive IP detected in HepG2 cells. Positive WB detected in HepG2 cells. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 86 kDa
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Tested applications
ELISA, WB, IP, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
ARNT antibody; ARNT protein antibody; bHLHe2 antibody; HIF 1 beta antibody; HIF 1beta antibody; HIF1 beta antibody; HIF1B antibody; HIF1BETA antibody; TANGO antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ARNT,HIF1B recombinant protein (Accession Number: BC060838). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IF: 1:50-1:500
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:108158(ARNT,HIF1B antibody) at dilution of 1:1500
IP Result of anti-ARNT,HIF1B (IP:Catalog No:108158, 4ug; Detection:Catalog No:108158 1:1000) with HepG2 cells lysate 3600ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:108158(ARNT,HIF1B Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
The antibody can recognize ARNT and ARNT2.
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References
- Liu Y, Zhang JB, Qin Y. PROX1 promotes hepatocellular carcinoma metastasis by way of up-regulating hypoxia-inducible factor 1α expression and protein stability. Hepatology (Baltimore, Md.). 58(2):692-705. 2013.
- Yang S, Yu M, Sun L. Interferon-γ-induced intestinal epithelial barrier dysfunction by NF-κB/HIF-1α pathway. Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research. 34(3):195-203. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"