-
Product Name
ARIH2 antibody
- Documents
-
Description
ARIH2 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HEK-293 cells, HeLa cells, human brain tissue, human colon tissue, human heart tissue, mouse embryo tissue, mouse kidney tissue. Observed molecular weight by Western-blot: 59 kDa
-
Tested applications
ELISA, WB, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
ARI 2 antibody; ARI2 antibody; ariadne homolog 2 (Drosophila) antibody; ARIH2 antibody; FLJ10938 antibody; FLJ33921 antibody; Protein ariadne 2 homolog antibody; TRIAD1 antibody; Triad1 protein antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of ARIH2 recombinant protein (Accession Number: XM_017005532). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
-
Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:108193(ARIH2 antibody) at dilution of 1:500
IP Result of anti-ARIH2 (IP:Catalog No:108193, 4ug; Detection:Catalog No:108193 1:500) with HeLa cells lysate 1200ug.
-
Background
ARIH2(Protein ariadne-2 homolog) is also named as ARI2, TRIAD1 and belongs to the RBR family and ariadne subfamily .It mediates 'Lys-48'-and 'Lys-63'-linked polyubiquitination and subsequent proteasomal degradation of modified proteins.
-
References
- Lin AE, Ebert G, Ow Y. ARIH2 is essential for embryogenesis, and its hematopoietic deficiency causes lethal activation of the immune system. Nature immunology. 14(1):27-33. 2013.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"