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Product Name
ARID3A antibody
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Description
ARID3A Rabbit Polyclonal antibody. Positive IP detected in K-562 cells. Positive WB detected in K-562 cells. Positive IHC detected in human colon cancer tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 75 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ARID3A antibody; BRIGHT antibody; Dead ringer like protein 1 antibody; DRIL1 antibody; DRIL3 antibody; DRX antibody; E2F binding protein 1 antibody; E2FBP1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ARID3A recombinant protein (Accession Number: XM_005259514). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:108191(ARID3A antibody) at dilution of 1:1500
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:108191(ARID3A antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:108191(ARID3A antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using ARID3A antibody Catalog No:108191 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-ARID3A (IP:Catalog No:108191, 4ug; Detection:Catalog No:108191 1:1000) with K-562 cells lysate 3200ug.
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Background
ARID3A is a nuclear matrix-associated transcription factor that stimulates immunoglobulin heavy chain (IgH) expression and Cyclin E1/E2F-dependent cell cycle progression. It activates IgH transcriptional initiation by binding to ATC-rich P sites within nuclear matrix attachment regions (MARs) flanking the IgH intronic enhancer (Emu) [PMID:17386101]. It is the founder of the 13-member (in humans) ARID (AT-Rich Interaction Domain) family, which share a highly conserved DNA binding domain, but function in diverse biological processes such as cell cycle regulated events, epigenetic post-translational modification, and chromatin remodeling [PMID:15927959,11959810,11283269].
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References
- Song M, Kim H, Kim WK, Hong SP, Lee C, Kim H. High expression of AT-rich interactive domain 3A (ARID3A) is associated with good prognosis in colorectal carcinoma. Annals of surgical oncology. 21 Suppl 4:S481-9. 2014.
- Madison BB, Jeganathan AN, Mizuno R. Let-7 Represses Carcinogenesis and a Stem Cell Phenotype in the Intestine via Regulation of Hmga2. PLoS genetics. 11(8):e1005408. 2015.
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