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Product Name
ARHGEF4 antibody
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Description
ARHGEF4 Rabbit Polyclonal antibody. Positive IHC detected in human brain tissue. Positive WB detected in mouse brain tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 72 kDa
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Tested applications
ELISA, IHC, WB, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
ARHGEF4 antibody; ASEF antibody; ASEF1 antibody; GEF4 antibody; KIAA1112 antibody; STM6 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_015320). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:108186(ARHGEF4 Antibody) at dilution of 1:1000
WB result of Catalog No:108186 (ARHGEF4 Antibody).
Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:108186(ARHGEF4 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:108186(ARHGEF4 Antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-ARHGEF4 (IP:Catalog No:108186, 4ug; Detection:Catalog No:108186 1:1000) with mouse brain tissue lysate 4000ug.
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Background
ARHGEF4, also named as KIAA1112 and Asef, acts as guanine nucleotide exchange factor (GEF) for RhoA and RAC1 GTPases. Binding of APC, ARHGEF4 may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion.(PMID: 10873612). This antibody is specific to ARHGEF4.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"