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Product Name
ARF5-Specific antibody
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Description
ARF5-Specific Rabbit Polyclonal antibody. Positive WB detected in mouse colon tissue, COLO 320 cells, human testis tissue, mouse brain tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 21 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ADP ribosylation factor 5 antibody; ARF5 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_001662). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:10-1:100
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Validations
mouse colon tissue were subjected to SDS PAGE followed by western blot with Catalog No:108248(ARF5-Specific antibody) at dilution of 1:300
Immunofluorescent analysis of HepG2 cells, using ARF5 antibody Catalog No:108248 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20kda protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. ARF4 and ARF5 are class II ARFs. (PMID: 7759471, PMID: 16042562). Their function are not fully described.The antibody is specific to ARF5.
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