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APOL1 antibody

Cat.#: 108032

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Product Information

  • Product Name
    APOL1 antibody
  • Documents
  • Description
    APOL1 Rabbit Polyclonal antibody. Positive WB detected in human blood tissue, A2780 cells, A549 cells, HeLa cells, HepG2 cells, MCF7 cells, SKOV-3 cells, SW 1990 cells. Positive IP detected in HepG2 cells. Positive IHC detected in human kidney tissue, human liver tissue, human pancreas tissue, human spleen tissue, human testis tissue. Positive IF detected in HepG2 cells. Positive FC detected in HepG2 cells. Observed molecular weight by Western-blot: 39 kDa
  • Tested applications
    ELISA, WB, IHC, IF, IP, FC
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    APO L antibody; APOL antibody; APOL I antibody; APOL1 antibody; Apolipoprotein L antibody; Apolipoprotein L I antibody; apolipoprotein L antibody; Apolipoprotein L1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of APOL1 recombinant protein (Accession Number: BC017331). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    human blood tissue were subjected to SDS PAGE followed by western blot with Catalog No:108032(APOL1 antibody) at dilution of 1:1000

    human blood tissue were subjected to SDS PAGE followed by western blot with Catalog No:108032(APOL1 antibody) at dilution of 1:1000

    IP Result of anti-APOL1 (IP:Catalog No:108032, 3ug; Detection:Catalog No:108032 1:500) with HepG2 cells lysate 6000ug.

    IP Result of anti-APOL1 (IP:Catalog No:108032, 3ug; Detection:Catalog No:108032 1:500) with HepG2 cells lysate 6000ug.

    Immunohistochemical of paraffin-embedded human kidney using Catalog No:108032(APOL1 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human kidney using Catalog No:108032(APOL1 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human kidney using Catalog No:108032(APOL1 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human kidney using Catalog No:108032(APOL1 antibody) at dilution of 1:50 (under 40x lens)

    Immunofluorescent analysis of HepG2 cells, using APOL1 antibody Catalog No: at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of HepG2 cells, using APOL1 antibody Catalog No: at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    1X10^6 HepG2 cells were stained with 0.2ug APOL1 antibody (Catalog No:108032, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 HepG2 cells were stained with 0.2ug APOL1 antibody (Catalog No:108032, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • Background
    Human apolipo-protein L1 (APOL1) is a minor component of plasma high density lipoprotein (HDL) particles, acting as an interacting protein of apolipoprotein A1 (ApoA1). The human ApoL protein family was thought to be predominantly involved in lipid transport and metabolism. APOL1 is also involved in host innate immunity against Trypanosoma parasites. Once activated, APOL1 can lyse the parasite and protect human from infection. Genetic variants in APOL1 gene, which are found in African ancestry with high frequency, associate with chronic kidney disease, like focal segmental glomerulosclerosis (FSGS), HIV-associated nephropathy (HIVAN), and hypertensive nephropathy. APOL1 share structural and functional similarities with proteins of the Bcl-2 family and may has roles in apoptosis and autophagy. It is notable that APOL1 exists only in human and a few other primate species, and mouse does not express an APOL1 orthologue. This antibody recognizes the endogenous APOL1 of 39-45 kDa in blood lysate.
  • References
    • Molina-Portela MP, Samanovic M, Raper J. Distinct roles of apolipoprotein components within the trypanosome lytic factor complex revealed in a novel transgenic mouse model. The Journal of experimental medicine. 205(8):1721-8. 2008.
    • Thomson R, Molina-Portela P, Mott H, Carrington M, Raper J. Hydrodynamic gene delivery of baboon trypanosome lytic factor eliminates both animal and human-infective African trypanosomes. Proceedings of the National Academy of Sciences of the United States of America. 106(46):19509-14. 2009.
    • Sun HY, Chen SF, Lai MD. Comparative proteomic profiling of plasma very-low-density and low-density lipoproteins. Clinica chimica acta; international journal of clinical chemistry. 411(5-6):336-44. 2010.
    • Thomson R, Genovese G, Canon C. Evolution of the primate trypanolytic factor APOL1. Proceedings of the National Academy of Sciences of the United States of America. 111(20):E2130-9. 2014.
    • Heneghan JF, Vandorpe DH, Shmukler BE. BH3 domain-independent apolipoprotein L1 toxicity rescued by BCL2 prosurvival proteins. American journal of physiology. Cell physiology. 309(5):C332-47. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"