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APOL1 antibody

Cat.#: 107065

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Product Information

  • Product Name
    APOL1 antibody
  • Documents
  • Description
    APOL1 Mouse Monoclonal antibody. Positive IHC detected in human liver tissue, human kidney tissue. Positive FC detected in HepG2 cells. Positive IP detected in human plasma tissue. Positive WB detected in human blood tissue. Observed molecular weight by Western-blot: 44 kDa
  • Tested applications
    ELISA, WB, IHC, FC, IP
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    APO L antibody; APOL antibody; APOL I antibody; APOL1 antibody; Apolipoprotein L antibody; Apolipoprotein L I antibody; apolipoprotein L antibody; Apolipoprotein L1 antibody
  • Isotype
    Mouse IgG2a
  • Preparation
    This antibody was obtained by immunization of APOL1 recombinant protein (Accession Number: BC017331). Purification method: Protein A purified.
  • Clonality
    Monoclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:5000-1:50000

    IP: 1:5000-1:50000

    IHC: 1:20-1:200

  • Validations

    human blood tissue were subjected to SDS PAGE followed by western blot with Catalog No:107065(APOL1 antibody) at dilution of 1:20000

    human blood tissue were subjected to SDS PAGE followed by western blot with Catalog No:107065(APOL1 antibody) at dilution of 1:20000

    Immunohistochemical of paraffin-embedded human liver using Catalog No:107065(APOL1 antibody) at dilution of 1:500 (under 10x lens)

    Immunohistochemical of paraffin-embedded human liver using Catalog No:107065(APOL1 antibody) at dilution of 1:500 (under 10x lens)

    IP Result of anti-APOL1 (IP:Catalog No:107065, 5ug; Detection:Catalog No:107065 1:10000) with human plasma tissue lysate 4000ug.

    IP Result of anti-APOL1 (IP:Catalog No:107065, 5ug; Detection:Catalog No:107065 1:10000) with human plasma tissue lysate 4000ug.

    1X10^6 HepG2 cells were stained with 0.2ug APOL1 antibody (Catalog No:107065, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

    1X10^6 HepG2 cells were stained with 0.2ug APOL1 antibody (Catalog No:107065, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

  • Background
    Human apolipo-protein L1 (APOL1) is a minor component of plasma high density lipoprotein (HDL) particles, acting as an interacting protein of apolipoprotein A1 (ApoA1). The human ApoL protein family was thought to be predominantly involved in lipid transport and metabolism. APOL1 is also involved in host innate immunity against Trypanosoma parasites. Once activated, APOL1 can lyse the parasite and protect human from infection. Genetic variants in APOL1 gene, which are found in African ancestry with high frequency, associate with chronic kidney disease, like focal segmental glomerulosclerosis (FSGS), HIV-associated nephropathy (HIVAN), and hypertensive nephropathy. APOL1 share structural and functional similarities with proteins of the Bcl-2 family and may has roles in apoptosis and autophagy. It is notable that APOL1 exists only in human and a few other primate species, and mouse does not express an APOL1 orthologue. This antibody recognizes the endogenous ApoL1 of 39-45 kDa in blood lysate.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"