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Product Name
APJ antibody
- Documents
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Description
APJ Rabbit Polyclonal antibody. Positive FC detected in SH-SY5Y cells. Positive IHC detected in human colon tissue. Positive WB detected in SH-SY5Y cells. Observed molecular weight by Western-blot: 43 kDa
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Tested applications
ELISA, WB, IHC, FC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
AGTRL1 antibody; Angiotensin receptor like 1 antibody; apelin receptor antibody; APJ antibody; APJR antibody; APLNR antibody; FLJ90771 antibody; G protein coupled receptor APJ antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of APJ recombinant protein (Accession Number: NM_005161). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with Catalog No:108142(APLNR Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human colon tissue slide using Catalog No:108142(APLNR Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human colon tissue slide using Catalog No:108142(APLNR Antibody) at dilution of 1:50 (under 10x lens)
1X10^6 SH-SY5Y cells were stained with 0.2ug APLNR antibody (Catalog No:108142, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
APJ, also named as APLNR and AGTRL1, belongs to the G-protein coupled receptor 1 family. It is a receptor for apelin coupled to G proteins that inhibit adenylate cyclase activity. APJ is an alternative coreceptor with CD4 for HIV-1 infection. It may be involved in the development of AIDS dementia. APJ is expressed abundantly in the corpus callosum and spinal cord.
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References
- Yuan ZS, Zhou YZ, Liao XB. Apelin attenuates the osteoblastic differentiation of aortic valve interstitial cells via the ERK and PI3-K/Akt pathways. Amino acids. 47(12):2475-82. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"