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Product Name
APAF1 antibody
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Description
APAF1 Rabbit Polyclonal antibody. Positive IHC detected in human heart tissue, human spleen tissue. Positive IF detected in A549 cells. Positive WB detected in HEK-293 cells, A549 cells, COLO 320 cells. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 130-142kd
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human, Rat; other species not tested.
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Alternative names
APAF 1 antibody; APAF1 antibody; CED4 antibody; DKFZp781B1145 antibody; KIAA0413 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of APAF1 recombinant protein (Accession Number: NM_181861). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:108128(APAF1 antibody) at dilution of 1:500
IP Result of anti-APAF1 (IP:Catalog No:108128, 4ug; Detection:Catalog No:108128 1:500) with HEK-293 cells lysate 2000ug.
Immunohistochemical of paraffin-embedded human heart using Catalog No:108128(APAF1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human heart using Catalog No:108128(APAF1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of A549 cells using Catalog No:108128(APAF1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
APAF1, also named as Apoptotic protease-activating factor 1 or KIAA0413, is a 1248 amino acid protein, which contains one CARD domain, contains one NB-ARC domain and Contains fifteen WD repeats. APAF1 localizes in the cytoplasm and is widely expressed in many tissues. Highest levels of expression is in adult spleen, peripheral blood leukocytes, in fetal brain, kidney and lung. Oligomeric APAF1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
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References
- Zhang S, Qi Y, Xu Y. Protective effect of flavonoid-rich extract from Rosa laevigata Michx on cerebral ischemia-reperfusion injury through suppression of apoptosis and inflammation. Neurochemistry international. 63(5):522-32. 2013.
- Shi J, Ren C, Liu H. An ESRG-interacting protein, COXII, is involved in pro-apoptosis of human embryonic stem cells. Biochemical and biophysical research communications. 460(2):130-5. 2015.
- Huang W, Liu J, Feng X. DLC-1 induces mitochondrial apoptosis and epithelial mesenchymal transition arrest in nasopharyngeal carcinoma by targeting EGFR/Akt/NF-κB pathway. Medical oncology (Northwood, London, England). 32(4):115. 2015.
- Zhang HT, Xue JH, Zhang ZW. Cold-inducible RNA-binding protein inhibits neuron apoptosis through the suppression of mitochondrial apoptosis. Brain research. 1622:474-83. 2015.
- Li S, Xu D, Guo J, Sun Y. Inhibition of cell growth and induction of inflammation by endosulfan in HUVEC-C cells. Environmental toxicology. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"