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Product Name
AP2B1 antibody
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Description
AP2B1 Rabbit Polyclonal antibody. Positive IF detected in Hela cells. Positive IP detected in K-562 cells. Positive WB detected in K-562 cells, C6 cells, HeLa cells. Observed molecular weight by Western-blot: 100 kDa-115 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ADTB2 antibody; AP 2 complex subunit beta antibody; AP105B antibody; AP2 BETA antibody; AP2B1 antibody; Beta 2 adaptin antibody; Beta adaptin antibody; CLAPB1 antibody; DKFZp781K0743 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of AP2B1 recombinant protein (Accession Number: XM_005257938). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IF: 1:10-1:100
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:108120(AP2B1 antibody) at dilution of 1:500
Immunofluorescent analysis of Hela cells, using AP2B1 antibody Catalog No:108120 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-AP2B1 (IP:Catalog No:108120, 4ug; Detection:Catalog No:108120 1:500) with K-562 cells lysate 3200ug.
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Background
AP2B1 is a component of the adaptor protein complex 2 (AP-2). AP complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP complexes are involved in the formation of clathrin-coated vesicles (CCVs) by recruiting the scaffold protein, clathrin. AP complexes also play a pivotal role in the cargo selection by recognizing the sorting signals within the cytoplasmic tail of integral membrane proteins. AP-2 works on the plasma membrane to internalize cargo in clathrin-mediated endocytosis.
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References
- Sohda M, Misumi Y, Ogata S. Trans-Golgi protein p230/golgin-245 is involved in phagophore formation. Biochemical and biophysical research communications. 456(1):275-81. 2015.
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