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Product Name
Anti-SULT2A1 antibody
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Description
Mouse monoclonal to SULT2A1
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Tested applications
ELISA, WB
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Species reactivity
Human SULT2A1 / STD
No cross-reactivity with Human SULT1B1 and E.coli cell lysate in WB and ELISA -
Alternative names
STD antibody; DHEAS antibody; DHEA-ST antibody; HST antibody; hSTa antibody; mSTa1 antibody; ST2 antibody; ST2A1 antibody; ST2A1 antibody; ST2A3 antibody; Std antibody; STD antibody; Sth1 antibody; SULT2A1 antibody; Sult2a1 antibody; HST antibody; hSTa antibody; DHEAS antibody; ST2A1 antibody; ST2A3 antibody; DHEA-ST antibody; Std antibody; Sth1 antibody; ST2A1 antibody; mSTa1 antibody; ST2 antibody
- Immunogen
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Isotype
Mouse IgG1
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Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SULT2A1 (rh SULT2A1; NP_003158.2; Ser 2-Glu 285). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/ml
ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human SULT2A1. The detection limit for Human SULT2A1 is approximately 0.039 ng/well.
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Validations
SULT2A1 / STD Antibody, Mouse MAb, Western blot
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Background
Hydroxysteroid sulfotransferase ( SULT2A1 ) is a key enzyme in the testicular and hepatic metabolism of 5alpha-androstenone, which is a major component of the off-odor and off-flavor in pork known as boar taint. Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. SULT2A1 is a sulfo-conjugating phase II enzyme expressed at very high levels in the liver and intestine, the two major first-pass metabolic tissues, and in the steroidogenic adrenal tissue. SULT2A1 acts preferentially on the hydroxysteroids dehydroepiandrosterone, testosterone/dihydrotestosterone, and pregnenolone and on cholesterol-derived amphipathic sterol bile acids.
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References
- Chatterjee, B. 2005, Methods Enzymol. 400:165-91.
- Liu,Y. et al., 2006, Chem Res Toxicol. 19 (11):1420-5.
- Sinclair, PA. et al., 2006, J Mol Endocrinol 36 (2):301-11.
- Yalcin, EB. et al., 2008, Drug Metab Lett 2 (3):198-204.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"