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Product Name
Anti-SPINK4 antibody
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Description
Rabbit polyclonal to SPINK4
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Tested applications
ELISA, IHC-P
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Species reactivity
Human SPINK4
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Alternative names
PEC-60 antibody; RP23-28I8.2 antibody; SPINK4 antibody; Spink4 antibody; MGC133107 antibody; MPGC60 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human SPINK4 (rh SPINK4; NP_055286.1; Met 1-Cys 86). SPINK4 specific IgG was purified by human SPINK4 affinity chromatography
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human SPINK4. The detection limit for Human SPINK4 is approximately 0.00975 ng/well.
IHC-P: 0.1-2 μg/mL
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Validations
SPINK4 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human SPINK4 in human pancreas with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
SPINK4 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human SPINK4 in human skin with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
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Background
Serine protease inhibitor Kazal-type 4, also known as Peptide PEC-60 homolog and SPINK4, is a secreted protein which contains one Kazal-like domain. SPINK4 is a member of the SPINK protein family. The gene family of serine protease inhibitors of the Kazal type (SPINK) are functional and positional candidate genes for celiac disease (CD). SPINK1 plays an important role in protecting the pancreas against excessive trypsinogen activation. It is a potent natural inhibitor of pancreatic trypsin activity. SPINK1 mutations are associated with the development of acute and chronic pancreatitis and have been detected in all forms of chronic pancreatitis. SPINK2 functions as a trypsin/acrosin inhibitor and is synthesized mainly in the testis and seminal vesicle where its activity is engaged in fertility. The SPINK2 protein contains a typical Kazal domain composed by six cysteine residues forming three disulfide bridges. SPINK9 was identified in human skin. Its expression was strong in palmar epidermis, but not detectable or very low in non palmoplantar skin.
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References
- Schneider, A. et al., 2004,Gastroenterol Clin North Am. 33 (4): 789-806.
- Wapenaar, MC. et al., 2007, Immunogenetics. 59 (5): 349-57.
- Brattsand, M. et al., 2009, J Invest Dermatol. 129 (7): 1656-65.
- Chen, T. et al., 2009, Proteins. 77 (1): 209-19.
- Noah, TK. et al., 2010, Exp Cell Res. 316 (3): 452-65.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"