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Anti-SMC1 (8E6) Mouse antibody

Cat.#: 168131

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Product Information

  • Product Name
    Anti-SMC1 (8E6) Mouse antibody
  • Documents
  • Description
    SMC1 (8E6) Mouse monoclonal antibody
  • Tested applications
    WB, IHC-P, FC
  • Species reactivity
    Human
  • Alternative names
    Chromosome segregation protein SmcB;DXS423E;KIAA0178;MGC138332;Sb1.8;Segregation of mitotic chromosomes 1;SMC protein 1A;SMC-1-alpha;SMC-1A;SMC1 (structural maintenance of chromosomes 1 yeast) like 1;SMC1;SMC1 structural maintenance of chromosomes 1 like antibody
  • Isotype
    Mouse IgG1
  • Preparation
    Antigen: Purified recombinant human SMC1A(N-terminus) protein fragments expressed in E.coli.
  • Clonality
    Monoclonal
  • Formulation
    Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4 150 mM NaCl) with 0.02% sodium azide 50% glycerol
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/1000

    ;FC: 1/100

  • Validations

    Western blot detection of SMC1A(N-terminus) in HelaNE,Jurkat and A431 cell lysates using SMC1A (N-terminus) mouse mAb (1:1000 diluted).Predicted band size: 143KDa.Observed band size: 143KDa.

    Western blot detection of SMC1A(N-terminus) in HelaNE,Jurkat and A431 cell lysates using SMC1A (N-terminus) mouse mAb (1:1000 diluted).Predicted band size: 143KDa.Observed band size: 143KDa.

    Flow Cytometry analysis of HeLa cells stained with SMC1A (N-terminus) (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Black line histogram represents the isotype control, normal mouse IgG.

    Flow Cytometry analysis of HeLa cells stained with SMC1A (N-terminus) (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Black line histogram represents the isotype control, normal mouse IgG.

    IHC of paraffin-embedded human colon using anti-SMC1A (N-terminus) mouse mAb diluted 1/500-1/1000.

    IHC of paraffin-embedded human colon using anti-SMC1A (N-terminus) mouse mAb diluted 1/500-1/1000.

  • Background
    Swiss-Prot Acc.Q14683.Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interac tion with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"