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Product Name
Anti-SEC61A Rabbit antibody
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Description
SEC61A Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC, IP
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant protein of human SEC61A
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/1000-1/5000
IHC: 1/20-1/50
ICC/IF: 1/50
FC: 1/20
IP: 1/20
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Validations
Western blot detection of SEC61A in K562,C6,3T3 cell lysates using SEC61A Rabbit pAb(1:1000 diluted).Predicted band size:52kDa.Observed band size:40kDa.
Immunocytochemistry of SEC61A(green) in Hela cells using SEC61A Rabbit pAb at dilution 1/50, and DAPI(blue)
Immunohistochemistry of SEC61A in paraffin-embedded Human lung cancer tissue using SEC61A Rabbit pAb at dilution 1/20
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Background
Swiss-Prot Acc.Q9H9S3.Appears to play a crucial role in the insertion of secretory and membrane polypeptides into the ER. It is required for assembly of membrane and secretory proteins. Found to be tightly associated with membrane-bound ribosomes, either directly or through adaptor proteins .
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"