Anti-Renin antibody

Cat.#: 102620

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Product Information

  • Product Name
    Anti-Renin antibody
  • Documents
  • Description
    Rabbit monoclonal to Renin
  • Tested applications
    ELISA, WB, IHC-P, IF, ICC/IF
  • Species reactivity
    Human Renin / REN / AngiotensinogenaseHas 20 % cross activity in ELISA with Mouse REN1 / Renin-1
  • Immunogen
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained from a rabbit immunized with purified, recombinant Human Renin (rh Renin; NP_000528.1; Met 1-Arg 406).
  • Clonality
    Monoclonal
  • Formulation
    0.2 μm filtered solution in PBS with 5% trehalose
  • Storage instructions
    This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
    Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
  • Applications

    WB: 10-20 μg/mL

    ELISA: 0.1-0.2 μg/mL

    This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human RENIN. The detection limit for Human RENIN is approximately 0.00245 ng/well.

    IHC-P: 1-10 μg/mL

    ICC/IF: 10-25 μg/mL

  • Validations

    Renin / REN / Angiotensinogenase Antibody, Rabbit MAb, Immunohistochemistry

    Renin / REN / Angiotensinogenase Antibody, Rabbit MAb, Immunohistochemistry

    Immunochemical staining of human RENIN in human kidney with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections).

    Renin / REN / Angiotensinogenase Antibody, Rabbit MAb, Western blot

    Renin / REN / Angiotensinogenase Antibody, Rabbit MAb, Western blot

    Renin / REN / Angiotensinogenase Antibody, Rabbit MAb, Immunofluorescence

    Renin / REN / Angiotensinogenase Antibody, Rabbit MAb, Immunofluorescence

    Immunofluorescence staining of Human Renin in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Renin monoclonal antibody (15 µg/ml) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

  • Background
    Mouse Renin-1, also known as Ren-1, Angiotensinogenase and Kidney renin, is a member of the peptidase A1 family. Renin-1 is synthesized by the juxtaglomerular cells of the kidney in response to decreased blood pressure and sodium concentration. androgen and thyroid hormones influence levels of Renin-1 in mouse submandibular gland (SMG) primarily by regulating the amount of Renin-1 mRNA available for translation. Renin-1 is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. It is expressed at relatively low levels in mouse SMG and kidney. Ren-2 is expressed at high levels in the mouse SMG and at very low levels, if at all, in the kidney. Ren-1 and Ren-2 are closely linked on mouse chromosome 1, show extensive homology in coding and noncoding regions and provide a model for studying the regulation of gene expression.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"