-
Product Name
Anti-REG3A antibody
- Documents
-
Description
Rabbit monoclonal to REG3A
-
Tested applications
ELISA, IHC-P
-
Species reactivity
Human REG3A
No cross-reactivity in ELISA with Human REG3G; Mouse REG3A; Human REG1B -
Alternative names
PAP antibody; REG-III antibody; AV063448 antibody; HIP antibody; PAP1 antibody; PAP-H antibody; PBCGF antibody; REG3 antibody; Reg3a antibody; REG3A antibody
- Immunogen
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human REG3A (rh REG3A; Q06141-1; Met 1-Asp 175).
-
Clonality
Monoclonal
-
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
-
Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human REG3A. The detection limit for Human REG3A is approximately 0.039 ng/well.
IHC-P: 1-10 μg/mL
-
Validations
REG3A Antibody, Rabbit MAb, Immunohistochemistry
Immunochemical staining of human REG3A in human pancreatic carcinoma with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections).
-
Background
Regenerating islet-derived protein 3-alpha, also known as Regenerating islet-derived protein III-alpha, REG-3-alpha, REG3A, and HIP, is secreted protein which contains one C-type lectin domain. REG3A is constitutively expressed in intestine, and is a pancreatic secretory protein that may be involved in cell proliferation or differentiation. It is overexpressed during the acute phase of pancreatitis and in some patients with chronic pancreatitis. REG3A and REG1A proteins are both involved in liver and pancreatic regeneration and proliferation. REG3A is also a stress protein involved in the control of bacterial proliferation. REG3A is down-regulated in most primary human gastric cancer cells, and might be useful in the diagnosis of gastric cancer. Additionally, REG3A is a target of beta-catenin signaling in Huh7 hepatoma cells. The REG1A and REG3A are downstream targets of the Wnt pathway during liver tumorigenesis.
-
References
- Cavard C, et al. (2006) Overexpression of regenerating islet-derived 1 alpha and 3 alpha genes in human primary liver tumors with beta-catenin mutations. Oncogene. 25(4): 599-608.
- Choi B, et al. (2007) Downregulation of regenerating islet-derived 3 alpha (REG3A) in primary human gastric adenocarcinomas. Exp Mol Med. 39(6): 796-804.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"