Anti-Pyruvate Dehydrogenase E1-alpha subunit Rabbit antibody

Cat.#: 167206

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Product Information

  • Product Name
    Anti-Pyruvate Dehydrogenase E1-alpha subunit Rabbit antibody
  • Documents
  • Description
    Pyruvate Dehydrogenase E1-alpha subunit Rabbit polyclonal antibody
  • Tested applications
    WB, IHC-P, ICC/IF, FC, IP
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    PDHA; PDHAD; PHE1A; PDHCE1A antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A synthetic peptide of human Pyruvate Dehydrogenase E1-alpha subunit
  • Clonality
    Polyclonal
  • Formulation
    Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
  • Storage instructions
    Store at -20°C. Stable for 12 months from date of receipt.
  • Applications

    WB: 1/1000-1/2000

    IHC: 1/20-1/100

    ICC/IF: 1/20-1/100

    FC: 1/20

    IP: 1/20-1/50

  • Validations

    Western blot detection of Pyruvate Dehydrogenase E1-alpha subunit in C6,3T3 cell lysates using Pyruvate Dehydrogenase E1-alpha subunit Rabbit pAb(1:1000 diluted).Predicted band size:43kDa.Observed band size:43kDa.

    Western blot detection of Pyruvate Dehydrogenase E1-alpha subunit in C6,3T3 cell lysates using Pyruvate Dehydrogenase E1-alpha subunit Rabbit pAb(1:1000 diluted).Predicted band size:43kDa.Observed band size:43kDa.

    Immunocytochemistry of Pyruvate Dehydrogenase E1-alpha subunit(green) in Hela cells using Pyruvate Dehydrogenase E1-alpha subunit Rabbit pAb at dilution 1/50, and DAPI(blue)

    Immunocytochemistry of Pyruvate Dehydrogenase E1-alpha subunit(green) in Hela cells using Pyruvate Dehydrogenase E1-alpha subunit Rabbit pAb at dilution 1/50, and DAPI(blue)

  • Background
    Swiss-Prot Acc.P08559.The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2, and thereby links the glycolytic pathway to the tricarboxylic cycle.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"