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Product Name
Anti-PRTFDC1 antibody
- Documents
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Description
Rabbit polyclonal to PRTFDC1
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Tested applications
ELISA, WB
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Species reactivity
Human PRTFDC1
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Alternative names
Gm13377 antibody; HHGP antibody; OTTMUSG00000011667 antibody; phosphoribosyl transferase domain containing 1 antibody; phosphoribosyltransferase domain containing 1 pseudogene antibody; phosphoribosyltransferase domain-containing protein 1 antibody; Prtfdc1 antibody; PRTFDC1 antibody; RP11-129O7.1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human PRTFDC1 (rh PRTFDC1; Q9NRG1-1; Met1-Val225). PRTFDC1 specific IgG was purified by Human PRTFDC1 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/ml
ELISA:0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human PRTFDC1. The detection limit for Human PRTFDC1 is 0.00245 ng/well.
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Validations
PRTFDC1 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
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Background
PRTFDC1 is a member of the purine/pyrimidine phosphoribosyltransferase family. It can bind GMP, IMP and alpha-D-5-phosphoribosyl 1-pyrophosphate (PRPP). The epigenetic silencing of PRTFDC1 by hypermethylation of the CpG island leads to a loss of PRTFDC1 function, which might be involved in squamous cell oral carcinogenesis. PRTFDC1 is a genetic modifier of HPRT-deficiency in the mouse and has important implications for unraveling the molecular etiology of lesch-Nyhan disease(LND). LND is a severe X-linked neurological disorder caused by a deficiency of hypoxanthine phosphoribosyltransferase. PRTFDC1 has a low, barely measurable phosphoribosyltransferase activity (in vitro).
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References
- Welin M. et al., 2010, FEBS J. 277 (23): 4920-30.
- Keebaugh AC. et al., 2011, PLoS One. 6 (7): e22381.
- Suzuki E. et al., 2007, Oncogene. 26 (57): 7921-32.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"