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Product Name
Anti-PFDN4 / PFD4 antibody
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Description
Rabbit polyclonal to PFDN4 / PFD4
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Tested applications
ELISA, IHC-P
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Species reactivity
Human PFDN4 / PFD4
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Alternative names
prefoldin subunit 4 antibody; PFDN4 antibody; C1 antibody; PFD4 antibody; prefoldin 4 antibody; protein C-1 antibody; prefoldin 4 antibody; Pfdn4 antibody; RP23-43J11.1 antibody; 0610025M10Rik antibody; 1810063B06Rik antibody; C-1 antibody; C1 antibody; Pfd4 antibody; C1 antibody; PFD4 antibody; C1 antibody; C-1 antibody; Pfd4 antibody; 0610025M10Rik antibody; 1810063B06Rik antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human PFDN4 / PFD4 (rh PFDN4 / PFD4; Q9NQP4; Met1-Ser134). PFDN4 / PFD4 specific IgG was purified by Human PFDN4 / PFD4 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA:0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human PFDN4 / PFD4. The detection limit for Human PFDN4 / PFD4 is < 0.039 ng/well.
IHC-P: 0.1-2 μg/ml
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Validations
PFDN4 / PFD4 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
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Background
PFDN4 is a member of the prefoldin beta subunit family. It is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. The complex, consisting of two alpha and four beta subunits, forms a double beta barrel assembly with six protruding coiled-coils. PFDN4 binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. PFDN4 also binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.
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References
- Iijima M, et al. (1996) Cloning of cDNA with possible transcription factor activity at the G1-S phase transition in human fibroblast cell lines. Acta Med Okayama. 50(2):73-7.
- Hartl FU, et al. (2002) Molecular chaperones in the cytosol: from nascent chain to folded protein. Science. 295(5561):1852-8.
- Vainberg I, et al. (1998) Prefoldin, a chaperone that delivers unfolded proteins to cytosolic chaperonin. Cell. 93(5):863-73.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"