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Product Name
Anti-MID1IP1 antibody
- Documents
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Description
Rabbit polyclonal to MID1IP1
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Tested applications
ELISA, WB, IP
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Species reactivity
Human MID1IP1
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Alternative names
MID1 interacting protein 1 antibody; MID1IP1 antibody; G12-like antibody; MIG12 antibody; S14R antibody; STRAIT11499 antibody; THRSPL antibody; MID1 interacting G12-like protein antibody; MID1 interacting protein 1 (gastrulation specific G12-like) antibody; gastrulation specific G12 homolog antibody; gastrulation-specific G12-like protein antibody; spot 14-R antibody; spot 14-related protein antibody; Mid1 interacting protein 1 (gastrulation specific G12-like (zebrafish)) antibody; Mid1ip1 antibody; RP23-130J1.1 antibody; 3110038L01Rik antibody; Mig12 antibody; S14R antibody; gastrulation-specific G12-like protein antibody; mid1-interacting G12-like protein antibody; mid1-interacting protein 1 antibody; protein STRAIT11499 homolog antibody; spot 14-R antibody; spot 14-related protein antibody; S14R antibody; Mig12 antibody; 3110038L01Rik antibody; S14R antibody; MIG12 antibody; THRSPL antibody; G12-like antibody; STRAIT11499 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human MID1IP1 (rh MID1IP1; Q9NPA3; Met1-His183). MID1IP1 specific IgG was purified by Human MID1IP1 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/ml
ELISA:0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human MID1IP1. The detection limit for Human MID1IP1 is < 0.039 ng/well.
IP: 1-4 μg/mg of lysate
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Validations
MID1IP1 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
MID1IP1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
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Background
MID1IP1 belongs to the SPOT14 family. It is a homodimer in the absence of THRSP. MID1IP1 interacts with ACACA and ACACB. It plays a role in the regulation of lipogenesis in liver. It up-regulates ACACA enzyme activity. MID1IP1 is required for efficient lipid biosynthesis, including triacylglycerol, diacylglycerol and phospholipid. MID1IP1 is involved in stabilization of microtubules. Its interaction with THRSP interferes with ACACA binding.
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References
- Aipoalani DL. et al., 2010, Endocrinology. 151 (5): 2071-7.
- Colbert CL. et al., 2010, Proc Natl Acad Sci. 107 (44): 18820-5.
- Inoue J. et al., 2011, Mol Endocrinol. 25 (6): 995-1005.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"