Anti-MEP1A antibody

Cat.#: 102318

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Product Information

  • Product Name
    Anti-MEP1A antibody
  • Documents
  • Description
    Rabbit polyclonal to MEP1A
  • Tested applications
    ELISA, WB
  • Species reactivity
    Mouse MEP1A
  • Alternative names
    Mep1a antibody; Mep1 antibody; Mep-1 antibody; Mep-1a antibody; AI098089 antibody; AW107200 antibody; PPHA antibody; AI098089 antibody; AW107200 antibody; Mep1 antibody; Mep-1 antibody; MEP1A antibody; Mep-1a antibody; PPHA antibody
  • Immunogen
  • Isotype
    Rabbit IgG
  • Preparation
    Produced in rabbits immunized with purified, recombinant Mouse MEP1A (rM MEP1A; NP_032611.2; Met 1-Arg 615). Total IgG was purified by Protein A affinity chromatography.
  • Clonality
    Polyclonal
  • Formulation
    0.2 μm filtered solution in PBS with 5% trehalose
  • Storage instructions
    This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
    Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
  • Applications

    WB: 10-20 μg/ml

    ELISA: 0.5-1.0 μg/mL

    This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect Mouse MEP1A. The detection limit for Mouse MEP1A is 0.00245 ng/well.

  • Validations

    MEP1A / Endopeptidase-2 Antibody, Rabbit PAb, Western blot

    MEP1A / Endopeptidase-2 Antibody, Rabbit PAb, Western blot

  • Background
    Meprin A subunit alpha, also known as MEP1A, and Endopeptidase-2, is a single-pass type I  membrane protein which belongs to the peptidase M12A family. MEP1A contains one EGF-like domain, one MAM domain, and one MATH domain. Meprins are unique plasma membrane and secreted metalloproteinases that are highly regulated at the transcriptional and post-translational levels. Meprin alpha and beta subunits are abundantly expressed in kidney and intestinal epithelial cells, are secreted into the urinary tract and intestinal lumen, and are found in leukocytes and cancer cells under certain conditions. Meprins are capable of proteolytically degrading extracellular matrix proteins, proteolytically processing bioactive proteins, and play a role in inflammatory processes. Meprin A and B are highly regulated, secreted and cell-surface homo- and hetero-oligomeric enzymes. Meprins are abundantly expressed in kidney and intestine. The multidomain alpha and beta subunits have high sequence identity. They have very different substrate specificities, oligomerization potentials and are differentially regulated. Meprin A appears to be an important therapeutic target and urinary excretion appears to be a potential biomarker of acute kidney injury ( AKI ).
  • References
    • Bertenshaw,GP. et al., 2002, Biol Chem. 383 (7-8):1175-83.
    • Bond, JS. et al., 2005, FEBS Lett. 579 (15): 3317-22.
    • Herzog, C. et al., 2007, Kidney Int. 71 (10): 1009-18.
    • Yura, RE. et al., 2009, Am J Physiol Renal Physiol. 296 (1): F135-44.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"