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Product Name
Anti-LSM1 antibody
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Description
Rabbit monoclonal to LSM1
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Tested applications
ELISA, WB
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Species reactivity
Human LSM1
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Alternative names
U6 small nuclear RNA associated (S. cerevisiae) antibody; U6 small nuclear RNA associated (S. cerevisiae) antibody; 2810025O06Rik antibody; cancer-associated Sm-like protein antibody; CASM antibody; CASM antibody; Lsm1 antibody; LSM1 antibody; LSM1 homolog antibody; LSM1 homolog antibody; small nuclear ribonuclear CaSm antibody; U6 snRNA-associated Sm-like protein LSm1 antibody; U6 snRNA-associated Sm-like protein LSm1 antibody; YJL124C antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human LSM1 (rh LSM1; O15116; Met1-Tyr133).
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 10-20 μg/ml
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL μg/ml with the appropriate secondary reagents to detect Human LSM1.
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Validations
LSM1 Antibody, Rabbit MAb, Western blot
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Background
LSM1 is a Sm-like protein. Sm-like proteins can be detected in a variety of organisms based on sequence homology with the Sm protein family. Sm-like proteins include the Sm sequence motif, which consists of two regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing. LSM1 has a role in replication-dependent histone mRNA degradation and binds specifically to the 3''-terminal U-tract of U6 snRNA. LSM1 also facilitates RNA protein interactions and structural modifications which are required during ribosomal subunit assembly.
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References
- Shimizu Y, et al. (1997) Lineage- and differentiation stage-specific expression of LSM-1 (LPAP), a possible substrate for CD45, in human hematopoietic cells. Am J Hematol. 54(1):1=11.
- Graber MW, et al. (1997) CaSm: an Sm-like protein that contributes to the transformed state in cancer cells. Cancer Res. 57(14):2961-5.
- Séraphin B, et al. (1999) Sm and Sm-like proteins assemble in two related complexes of deep evolutionary origin. EMBO J. 18(12):3451-62.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"