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Product Name
Anti-IKB beta (4B11) Mouse antibody
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Description
IKB beta (4B11) Mouse monoclonal antibody
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Tested applications
WB
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Species reactivity
Human
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant fragment of human NFKBIB expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Ascitic fluid containing 0.03% sodium azide.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
ELISA: 1/10000
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Validations
Western blot detection of IκBβ in Jurkat cell lysates using IκBβ mouse mAb (1:1000 diluted).Predicted band size:48KDa.Observed band size:48KDa.
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Background
Swiss-Prot Acc.Q15653.Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further IKBA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.Tissue specificity: Expressed in all tissues examined.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"