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Product Name
Anti-Hsp60 (6C8) Mouse antibody
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Description
Hsp60 (6C8) Mouse monoclonal antibody
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Tested applications
WB, ICC/IF, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
60 kDa chaperonin;60 kDa heat shock protein mitochondrial;60 kDa heat shock protein antibody; mitochondrial;CH60_HUMAN;Chaperonin 60;Chaperonin;Chaperonin antibody; 60-KD;CPN 60;CPN60;fa04a05;GROEL;GroEL Homolog;Heat shock 60kD protein 1 chaperonin;Heat shock 60kDa protein antibody
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Isotype
Mouse IgG2b
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Preparation
Antigen: Purified recombinant human Hsp60 protein fragments expressed in E.coli.
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Clonality
Monoclonal
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Formulation
Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
;ICC: 1/100
;IP: 1/100
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Validations
Western blot detection of Hsp60 in Hela,Raji,Jurkat,C6,C2C12 and 3T3 cell lysates using Hsp60 mouse mAb (1:1000 diluted).Predicted band size:60KDa.Observed band size:60KDa.
Immunocytochemistry staining of HeLa cells fixed with -20u2103 Methanol and using Hsp60 mouse mAb (dilution 1:100).
Immunoprecipitation analysis of Hela cell lysates using Hsp60 mouse mAb.
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Background
Swiss-Prot Acc.P10809.Implicated in mitochondrial protein import and macromolecular assembly.May facilitate the correct folding of imported proteins.May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"