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Product Name
Anti-glutaredoxin-1 / GRX1 / GLRX antibody
- Documents
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Description
Rabbit polyclonal to glutaredoxin-1 / GRX1 / GLRX
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Tested applications
ELISA, WB, IHC-P
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Species reactivity
Human glutaredoxin-1 / GRX1 / GLRX
No cross-reactivity in ELISA with Human cell lysate (293 cell line) -
Alternative names
guanosine monophosphate reductase antibody; GMPR antibody; RP3-467D16.2 antibody; GMPR1 antibody; GMP reductase 1 antibody; guanine monophosphate reductase antibody; guanosine 5'-monophosphate oxidoreductase 1 antibody; guanosine monophosphate reductase 1 antibody; guanosine monophosphate reductase antibody; Gmpr antibody; 2310004P21Rik antibody; AV028449 antibody; GMP reductase 1 antibody; guanosine 5'-monophosphate oxidoreductase 1 antibody; guanosine monophosphate reductase 1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human glutaredoxin-1 / GRX1 / GLRX (rh glutaredoxin-1 / GRX1 / GLRX; P35754; Met1-Gln106). glutaredoxin-1 / GRX1 / GLRX specific IgG was purified by Human glutaredoxin-1 / GRX1 / GLRX affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 10-20 μg/ml
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human glutaredoxin-1 / GRX1 / GLRX. The detection limit for Human glutaredoxin-1 / GRX1 / GLRX is < 0.039 ng/well.
IHC-P: 0.05-0.5 μg/ml
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Validations
glutaredoxin-1 / GRX1 / GLRX Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
glutaredoxin-1 / GRX1 / GLRX Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human GLRX in human placenta with rabbit polyclonal antibody (0.1 µg/mL, formalin-fixed paraffin embedded sections).
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Background
Glutaredoxin-1, also known as GRX1 and GLRX, belongs to the glutaredoxin family. Glutaredoxins are small redox enzymes that use glutathione as a cofactor. Glutaredoxins are oxidized by substrates, and reduced non-enzymatically by glutathione. Glutaredoxin-1 functions as an electron carrier in the glutathione-dependent synthesis of deoxyribonucleotides by the enzyme ribonucleotide reductase. Glutaredoxin-1 exists in either a reduced or an oxidized form. Glutaredoxins function as electron carriers in the glutathione-dependent synthesis of deoxyribonucleotides by the enzymeribonucleotide reductase.
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References
- Holmgren A. et al., 1988, FEMS Microbiol Rev. 4 (4): 271-97.
- Holmgren A. 1988, Biochem Soc Trans. 16 (2): 95-6.
- Holmgren A. 1989, J Biol Chem. 264 (24): 13963-6.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"