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Product Name
Anti-ETHE1 / HSCO antibody
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Description
Rabbit polyclonal to ETHE1 / HSCO
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Tested applications
ELISA, WB, IHC-P, IP
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Species reactivity
Human ETHE1 / HSCO
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Alternative names
mitochondrial antibody; ethylmalonic encephalopathy 1 antibody; ETHE1 antibody; HSCO antibody; YF13H12 antibody; ethylmalonic encephalopathy protein 1 antibody; hepatoma subtracted clone one protein antibody; protein ETHE1 antibody; ethylmalonic encephalopathy 1 antibody; Ethe1 antibody; 0610025L15Rik antibody; Hsco antibody; ethylmalonic encephalopathy protein 1 homolog antibody; hepatoma subtracted clone one protein antibody; protein ETHE1 antibody; mitochondrial antibody; HSCO antibody; YF13H12 antibody; Hsco antibody; 0610025L15Rik antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human ETHE1 / HSCO (rh ETHE1 / HSCO; O95571; Leu13-Ala254). ETHE1 / HSCO specific IgG was purified by Human ETHE1 / HSCO affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/ml
ELISA:0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human ETHE1 / HSCO. The detection limit for Human ETHE1 / HSCO is < 0.039 ng/well.
IHC-P: 0.1-1 μg/ml
IP: 1-4 μg/mg of lysate
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Validations
ETHE1 / HSCO Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
ETHE1 / HSCO Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
ETHE1 / HSCO Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
ETHE1 / HSCO Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
ETHE1 / HSCO Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
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Background
ETHE1, also known as HSCO, is a sulfur dioxygenase that localizes within the mitochondrial matrix. ETHE1 probably plays an important role in metabolic homeostasis in mitochondria. It may also function as a nuclear-cytoplasmic shuttling protein that binds transcription factor RELA/NFKB3 in the nucleus and exports it to the cytoplasm. ETHE1 can suppresses p53-induced apoptosis by preventing nuclear localization of RELA. Mutations in ETHE1 gene result in ethylmalonic encephalopathy. Ethylmalonic encephalopathy is an autosomal recessive, invariably fatal disorder characterized by early-onset encephalopathy, microangiopathy, chronic diarrhea, defective cytochrome c oxidase (COX) in muscle and brain, high concentrations of C4 and C5 acylcarnitines in blood and high excretion of ethylmalonic acid in urine.
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References
- Higashitsuji. et al., 2002, Cancer Cell. 2 (4): 335-46.
- McCoy JG. et al., 2007, Acta Crystallogr D Biol Crystallogr. 62 (9): 964-70.
- Mehrle A. et al., 2006, Nucleic Acids Res. 34: D415-8.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"